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HIF-1 alpha Polyclonal antibody

HIF-1 alpha Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

FC, IF, IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 20960-1-AP

Print datasheet

Synonyms

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF-1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8



经过测试的应用

Positive WB detected inCobalt Chloride treated HeLa cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman thyroid cancer tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells
Positive FC detected inHeLa cells

Samples need to be treated with hypoxia stimulation.

Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20960-1-AP targets HIF-1 alpha in WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications Cell treatment, ChIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, goat, chicken, pig
Immunogen HIF-1 alpha fusion protein Ag15198 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF-1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8
Calculated Molecular Weight 826 aa, 93 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC012527
Gene Symbol HIF1A
Gene ID (NCBI) 3091
RRIDAB_10732601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16665
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

实验方案

Product Specific Protocols
WB protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
IHC protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
IF protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
IP protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Gastroenterology

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Authors - Decai Wang
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
IF

Cancer Cell

Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

Authors - Agnieszka Chryplewicz

Nat Cell Biol

A single-cell transcriptomic landscape of the lungs of patients with COVID-19.

Authors - Si Wang
WB

ACS Nano

Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling

Authors - Bide Tong
human

Mol Cell

KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis.

Authors - Xu Qian