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- KD/KO Validated
IL-18 Polyclonal antibody
IL-18 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF/ICC, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
282
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, NIH/3T3 cells, DU 145 cells, HaCaT cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10663-1-AP targets IL-18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | IL-18 fusion protein Ag1063 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | IL-18 |
Synonyms | IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma |
Calculated Molecular Weight | 193 aa, 22 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC007461 |
Gene Symbol | IL-18 |
Gene ID (NCBI) | 3606 |
RRID | AB_2123636 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14116 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IL-18 antibody 10663-1-AP | Download protocol |
IF protocol for IL-18 antibody 10663-1-AP | Download protocol |
IP protocol for IL-18 antibody 10663-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv FUNDC1 interacts with FBXL2 to govern mitochondrial integrity and cardiac function through an IP3R3-dependent manner in obesity. | ||
Nat Commun IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence. | ||
J Pineal Res Melatonin inhibits atherosclerosis progression via Galectin-3 downregulation to enhance autophagy and inhibit inflammation | ||
Aging Dis Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice. | ||
Proc Natl Acad Sci U S A Telomere dysfunction instigates inflammation in inflammatory bowel disease. |