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IL-1 beta Polyclonal antibody

IL-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16806-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (7)

应用

WB, IHC, IF/ICC, ELISA and More (1)

Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inLPS and Protein transport inhibitor treated THP-1 cells, human adrenal gland tissue, A431 cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inLPS treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16806-1-AP targets IL-1 beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, ELISA
经测试反应性 human
文献引用反应性human, pig, rabbit, canine, zebrafish, bovine, sheep, duck
免疫原 IL-1 beta fusion protein Ag10295 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 1, beta
别名 Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta
计算分子量 269 aa, 31 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC008678
基因名称 IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
RRIDAB_10646432
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01584
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

实验方案

Product Specific Protocols
WB protocol for IL-1 beta antibody 16806-1-APDownload protocol
IHC protocol for IL-1 beta antibody 16806-1-APDownload protocol
IF protocol for IL-1 beta antibody 16806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Immunity

Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation.

Authors - Yiqing Yan
zebrafishIF

Cell

Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System.

Authors - Yunlu Zhu
WB

Trends Biotechnol

Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapy

Authors - Kaifeng Li
WB,IF

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
zebrafishIF

Nat Commun

Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages in zebrafish spinal cord regeneration.

Authors - Themistoklis M Tsarouchas

Nat Commun

Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells.

Authors - Akiko Takahashi
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