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IL-6 Polyclonal antibody

IL-6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 21865-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (5)

应用

WB, IP, ELISA and More (2)

IL6, Interleukin 6, Interferon beta-2, IL 6, IFN-beta-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLPS and Protein transport inhibitor treated HUVEC cells
Positive IP detected inLPS and Protein transport inhibitor treated HUVEC cells
Positive IF/ICC detected inLPS and Brefeldin A treated HUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21865-1-AP targets IL-6 in WB, IHC, IP, Neutralization, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, Neutralization, ELISA
经测试反应性 human
文献引用反应性human, pig, rabbit, canine, bovine, goat
免疫原 IL-6 fusion protein Ag10565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 6 (interferon, beta 2)
别名 IL6, Interleukin 6, Interferon beta-2, IL 6, IFN-beta-2
计算分子量 212 aa, 24 kDa
观测分子量20 kDa
GenBank蛋白编号BC015511
基因名称 IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
RRIDAB_11142677
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05231
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

实验方案

Product Specific Protocols
WB protocol for IL-6 antibody 21865-1-APDownload protocol
IF protocol for IL-6 antibody 21865-1-APDownload protocol
IP protocol for IL-6 antibody 21865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas

J Hematol Oncol

FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion

Authors - Zeda Zhao
IF

Cancer Cell

Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.

Authors - Yansen Xiao

Bioact Mater

Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy

Authors - Xin Huang
IHC

Bioact Mater

A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds

Authors - Shunlai Shang

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
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