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mTOR Monoclonal antibody
mTOR Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat and More (8)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
331
CloneNo.
1G11A3
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, ROS1728 cells, Caco-2 cells, HepG2 cells, Jurkat cells, NCI-H1299 cells, THP-1 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66888-1-Ig targets mTOR in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, PLA |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, hamster, goat, zebra finches |
| Immunogen | mTOR fusion protein Ag28395 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
| Synonyms | FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1 |
| Calculated Molecular Weight | 289 kDa |
| Observed Molecular Weight | 250-289 kDa |
| GenBank Accession Number | NM_004958 |
| Gene Symbol | mTOR |
| Gene ID (NCBI) | 2475 |
| RRID | AB_2882219 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42345 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for mTOR antibody 66888-1-Ig | Download protocol |
| IHC protocol for mTOR antibody 66888-1-Ig | Download protocol |
| IF protocol for mTOR antibody 66888-1-Ig | Download protocol |
| IP protocol for mTOR antibody 66888-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Mol Cell Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells | ||
Mol Cancer circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. | ||
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Nat Commun Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus. |
