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CoraLite®594-conjugated mTOR Monoclonal antibody

mTOR Monoclonal Antibody for IF/ICC
Cat No. CL594-66888

产品说明书

CloneNo. 1G11A3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat and More (2)

应用

IF/ICC and More (1)

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66888-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

CL594-66888 targets mTOR in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
文献引用应用IF
经测试反应性 Human, Mouse, Rat
文献引用反应性human, mouse
免疫原 mTOR fusion protein Ag28395 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 FK506 binding protein 12-rapamycin associated protein 1
别名 FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1
计算分子量 289 kDa
观测分子量 250-289 kDa
GenBank蛋白编号NM_004958
基因名称 mTOR
Gene ID (NCBI) 2475
RRIDAB_2883633
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42345
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).

实验方案

Product Specific Protocols
IF protocol for CL594 mTOR antibody CL594-66888Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Genet Med

De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood

Authors - Margot R F Reijnders
mouseIF

bioRxiv

Monocytes reprogrammed by 4-PBA potently contribute to the resolution of inflammation and atherosclerosis

Authors - Shuo Geng
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