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- KD/KO Validated
mTOR Monoclonal antibody
mTOR Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat and More (8)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
371
CloneNo.
1G11A3
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, ROS1728 cells, Caco-2 cells, HepG2 cells, Jurkat cells, NCI-H1299 cells, THP-1 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66888-1-Ig targets mTOR in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, PLA |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse, rat, pig, chicken, hamster, goat, zebra finches |
Immunogen | mTOR fusion protein Ag28395 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
Synonyms | FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1 |
Calculated Molecular Weight | 289 kDa |
Observed Molecular Weight | 250-289 kDa |
GenBank Accession Number | NM_004958 |
Gene Symbol | mTOR |
Gene ID (NCBI) | 2475 |
RRID | AB_2882219 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P42345 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for mTOR antibody 66888-1-Ig | Download protocol |
IHC protocol for mTOR antibody 66888-1-Ig | Download protocol |
IF protocol for mTOR antibody 66888-1-Ig | Download protocol |
IP protocol for mTOR antibody 66888-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Nat Commun Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice | ||
Mol Cell Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells | ||
Mol Cancer circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. | ||
Cell Rep Med Reprograming immunosuppressive microenvironment by eIF4G1 targeting to eradicate pancreatic ductal adenocarcinoma |