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验证数据展示

  • WB analysis using 12143-1-AP

    WB analysis using 12143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 12143-1-AP

    IHC staining of human tonsillitis using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IHC staining of human tonsillitis using 12143-1-AP

    IHC staining of human tonsillitis using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IP experiment of A431 using 12143-1-AP

    IP experiment of A431 using 12143-1-AP

    IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:1000) with A431 cells lysate 1200 ug.

  • IF Staining of A431 using 12143-1-AP

    IF Staining of A431 using 12143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p63 antibody (12143-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of A431 using 12143-1-AP

    FC experiment of A431 using 12143-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human p63 (12143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of human lung cancer using 12143-1-AP

    IF Staining of human lung cancer using 12143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

p63 Polyclonal antibody

p63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 12143-1-AP

产品说明书

引用文献(64)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (3)

别名

TP63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, human heart tissue, mouse heart tissue, U-937 cells, Apoptosised HeLa cells, HaCaT cells, PC-12 cells, mouse thymus tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman tonsillitis tissue, human bowen disease tissue, human lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy® TP63 Ready-To-Use IHC Kit. TP63 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12143-1-AP targets p63 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag2791

Product name: Recombinant human TP63 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 381-680 aa of BC039815

Sequence: NTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor protein p63
别名 TP63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63
计算分子量 680 aa, 77 kDa
观测分子量 63-68 kDa
GenBank蛋白编号BC039815
基因名称 TP63
Gene ID (NCBI) 8626
RRIDAB_10597397
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3D4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).

实验方案

Product Specific Protocols
FC protocol for p63 antibody 12143-1-APDownload protocol
IF protocol for p63 antibody 12143-1-APDownload protocol
IHC protocol for p63 antibody 12143-1-APDownload protocol
IP protocol for p63 antibody 12143-1-APDownload protocol
WB protocol for p63 antibody 12143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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