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  • Featured Product
  • KD/KO Validated

p63 Polyclonal antibody

p63 Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 12143-1-AP

Print datasheet

Synonyms

TP63, tumor protein p63, Tumor protein 63, Transformation-related protein 63, TP 63

验证数据展示

  • WB analysis using 12143-1-AP

    WB analysis using 12143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 12143-1-AP

    IHC staining of human tonsillitis using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IHC staining of human tonsillitis using 12143-1-AP

    IHC staining of human tonsillitis using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IP experiment of A431 using 12143-1-AP

    IP experiment of A431 using 12143-1-AP

    IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:1000) with A431 cells lysate 1200 ug.

  • IHC staining of human bowen disease using 12143-1-AP

    IHC staining of human bowen disease using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12143-1-AP (p63 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IHC staining of human bowen disease using 12143-1-AP

    IHC staining of human bowen disease using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12143-1-AP (p63 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IF Staining of A431 using 12143-1-AP

    IF Staining of A431 using 12143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p63 antibody (12143-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of A431 using 12143-1-AP

    FC experiment of A431 using 12143-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human p63 (12143-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of human lung cancer using 12143-1-AP

    IF Staining of human lung cancer using 12143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, human heart tissue, mouse heart tissue, U-937 cells, Apoptosised HeLa cells, HaCaT cells, PC-12 cells, mouse thymus tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman tonsillitis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TP63 Ready-To-Use IHC Kit. TP63 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12143-1-AP targets p63 in WB, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen p63 fusion protein Ag2791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor protein p63
Synonyms TP63, tumor protein p63, Tumor protein 63, Transformation-related protein 63, TP 63
Calculated Molecular Weight 680 aa, 77 kDa
Observed Molecular Weight 63-68 kDa
GenBank Accession NumberBC039815
Gene Symbol TP63
Gene ID (NCBI) 8626
RRIDAB_10597397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3D4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

实验方案

Product Specific Protocols
WB protocol for p63 antibody 12143-1-APDownload protocol
IHC protocol for p63 antibody 12143-1-APDownload protocol
IF protocol for p63 antibody 12143-1-APDownload protocol
IP protocol for p63 antibody 12143-1-APDownload protocol
FC protocol for p63 antibody 12143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
humanWB,IP,ChIP

Nucleic Acids Res

Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.

Authors - Yongsheng Huang
  • KD Validated
View Article
humanIF

Cell Rep

A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma

Authors - Yue Li
View Article
mouse

Oncogene

TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice.

Authors - Samera H Hamad
View Article
humanIF

Elife

Chloride channels regulate differentiation and barrier functions of the mammalian airway.

Authors - Mu He
View Article
human

Oncogene

Circulating IGF-1 promotes prostate adenocarcinoma via FOXO3A/BIM signaling in a double-transgenic mouse model.

Authors - Shuang Wang
View Article