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YAP1 Polyclonal antibody

YAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 13584-1-AP

Print datasheet

Synonyms

Yes-associated protein 1, yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog

验证数据展示

  • WB analysis using 13584-1-AP

    WB analysis using 13584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13584-1-AP

    IHC staining of human liver cancer using 13584-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YAP1 antibody included
  • IP experiment of NIH/3T3 using 13584-1-AP

    IP experiment of NIH/3T3 using 13584-1-AP

    IP result of anti-YAP1 (IP:13584-1-AP, 3ug; Detection:13584-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

  • IF Staining of HepG2 using 13584-1-AP

    IF Staining of HepG2 using 13584-1-AP

    Immunofluorescent analysis of HepG2 cells using 13584-1-AP (YAP1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • WB analysis using 13584-1-AP

    WB analysis using 13584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13584-1-AP

    WB analysis using 13584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13584-1-AP

    WB analysis using 13584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13584-1-AP

    WB analysis using 13584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of human lung cancer using 13584-1-AP

    IF Staining of human lung cancer using 13584-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human lung cancer using 13584-1-AP

    IF Staining of human lung cancer using 13584-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 13584-1-AP

    IF Staining of HepG2 using 13584-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, BGC-823 cells, MCF-7 cells, HepG2 cells, SGC-7901 cells, mouse liver tissue, rat liver tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YAP1 Ready-To-Use IHC Kit. YAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13584-1-AP targets YAP1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, zebrafish
Immunogen YAP1 fusion protein Ag4510 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Yes-associated protein 1, 65kDa
Synonyms Yes-associated protein 1, yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog
Calculated Molecular Weight 504 aa, 54 kDa
Observed Molecular Weight65-75 kDa
GenBank Accession NumberBC038235
Gene Symbol YAP1
Gene ID (NCBI) 10413
RRIDAB_2218915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46937
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers (PMID: 31613226, 32488048, 33520338). Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (PMID: 20048001).This antibody is a rabbit polyclonal antibody. The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).

实验方案

Product Specific Protocols
WB protocol for YAP1 antibody 13584-1-APDownload protocol
IHC protocol for YAP1 antibody 13584-1-APDownload protocol
IF protocol for YAP1 antibody 13584-1-APDownload protocol
IP protocol for YAP1 antibody 13584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Mechanisms of stretch-mediated skin expansion at single-cell resolution.

Authors - Mariaceleste Aragona
View Article
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
IF

Nature

Fat1 deletion promotes hybrid EMT state, tumour stemness and metastasis.

Authors - Ievgenia Pastushenko
View Article
mouseWB

Nature

YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING.

Authors - Hanna Lucie Sladitschek-Martens
View Article
humanIF

Nature

The SWI/SNF complex is a mechanoregulated inhibitor of YAP and TAZ.

Authors - Lei Chang
View Article
mouseIF

Nat Mater

Reprogramming normal cells into tumour precursors requires ECM stiffness and oncogene-mediated changes of cell mechanical properties.

Authors - Tito Panciera
View Article