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  • Featured Product
  • KD/KO Validated

RCHY1 Monoclonal antibody

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C11C11

Cat no : 67856-1-Ig

Print datasheet

Synonyms

ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

验证数据展示

  • WB analysis of HEK-293 using 67856-1-Ig

    WB analysis of HEK-293 using 67856-1-Ig

    WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells.

  • WB analysis using 67856-1-Ig

    WB analysis using 67856-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 67856-1-Ig

    IHC staining of human breast cancer using 67856-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67856-1-Ig

    IHC staining of human breast cancer using 67856-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 67856-1-Ig

    IF Staining of A549 using 67856-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67856-1-Ig

    IF Staining of HeLa using 67856-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, Daudi cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67856-1-Ig targets RCHY1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen RCHY1 fusion protein Ag4942 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ring finger and CHY zinc finger domain containing 1
Synonyms ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363
Calculated Molecular Weight 261 aa, 30 kDa
GenBank Accession NumberBC047393
Gene Symbol RCHY1
Gene ID (NCBI) 25898
RRIDAB_2918614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

实验方案

Product Specific Protocols
WB protocol for RCHY1 antibody 67856-1-IgDownload protocol
IHC protocol for RCHY1 antibody 67856-1-IgDownload protocol
IF protocol for RCHY1 antibody 67856-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

An AKT1-and TRIM21-mediated phosphodegron controls proteasomal degradation of HuR enabling cell survival under heat shock

Authors - Sharanya Nag
  • KD Validated
View Article