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  • Featured Product
  • KD/KO Validated

RCHY1 Monoclonal antibody, PBS Only

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C11C11

Cat no : 67856-1-PBS

Print datasheet

Synonyms

ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

验证数据展示

  • WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

    WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

    WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

    WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

    IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

    IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

产品信息

67856-1-PBS targets RCHY1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen RCHY1 fusion protein Ag4942 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ring finger and CHY zinc finger domain containing 1
Synonyms ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363
Calculated Molecular Weight 261 aa, 30 kDa
GenBank Accession NumberBC047393
Gene Symbol RCHY1
Gene ID (NCBI) 25898
RRIDAB_2918614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PM5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.