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验证数据展示

  • WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

    WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

    WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

    WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

    IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

    IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RCHY1 Monoclonal antibody, PBS Only

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67856-1-PBS

产品说明书

CloneNo. 2C11C11

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67856-1-PBS targets RCHY1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 RCHY1 fusion protein Ag4942 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ring finger and CHY zinc finger domain containing 1
别名 ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363
计算分子量 261 aa, 30 kDa
GenBank蛋白编号BC047393
基因名称 RCHY1
Gene ID (NCBI) 25898
RRIDAB_2918614
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96PM5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.