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Occludin Recombinant antibody

Occludin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

8B2

Cat no : 80545-1-RR

Print datasheet

Synonyms

OCLN, 8B2

验证数据展示

  • WB analysis using 80545-1-RR

    WB analysis using 80545-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 80545-1-RR

    IF Staining of MCF-7 using 80545-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IP experiment of HUVEC using 80545-1-RR

    IP experiment of HUVEC using 80545-1-RR

    IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug.

  • Affinity and Kinetic Characterization of 80545-1-RR

    Affinity and Kinetic Characterization of 80545-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HaCaT cells, pig colon tissue, mouse colon tissue, HUVEC cells, U2OS cells
Positive IP detected inHUVEC cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80545-1-RR targets Occludin in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, Mouse, Pig
Immunogen Occludin fusion protein Ag26173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name occludin
Synonyms OCLN, 8B2
Calculated Molecular Weight 522 aa, 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberBC029886
Gene Symbol Occludin
Gene ID (NCBI) 4950
RRIDAB_2918901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

实验方案

Product Specific Protocols
WB protocol for Occludin antibody 80545-1-RRDownload protocol
IF protocol for Occludin antibody 80545-1-RRDownload protocol
IP protocol for Occludin antibody 80545-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols