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Occludin Recombinant antibody, PBS Only

Occludin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8B2

Cat no : 80545-1-PBS

Print datasheet

Synonyms

OCLN, 8B2

验证数据展示

  • WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

    WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80545-1-RR (same clone as 80545-1-PBS)

    IF Staining of MCF-7 using 80545-1-RR (same clone as 80545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

  • IP experiment of HUVEC using 80545-1-RR (same clone as 80545-1-PBS)

    IP experiment of HUVEC using 80545-1-RR (same clone as 80545-1-PBS)

    IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80545-1-PBS

    Affinity and Kinetic Characterization of 80545-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

产品信息

80545-1-PBS targets Occludin in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Pig
Immunogen Occludin fusion protein Ag26173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name occludin
Synonyms OCLN, 8B2
Calculated Molecular Weight 522 aa, 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberBC029886
Gene Symbol Occludin
Gene ID (NCBI) 4950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16625
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).