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验证数据展示

  • WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

    WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80545-1-RR (same clone as 80545-1-PBS)

    IF Staining of MCF-7 using 80545-1-RR (same clone as 80545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

  • IP experiment of HUVEC using 80545-1-RR (same clone as 80545-1-PBS)

    IP experiment of HUVEC using 80545-1-RR (same clone as 80545-1-PBS)

    IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80545-1-PBS

    Affinity and Kinetic Characterization of 80545-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

Occludin Recombinant antibody, PBS Only

Occludin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 80545-1-PBS

产品说明书

CloneNo. 8B2

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Pig

应用

WB, IF/ICC, IP, Indirect ELISA

别名

OCLN, 8B2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

80545-1-PBS targets Occludin in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Pig samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Pig
免疫原 Occludin fusion protein Ag26173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 occludin
别名 OCLN, 8B2
计算分子量 522 aa, 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC029886
基因名称 Occludin
Gene ID (NCBI) 4950
RRIDAB_2918901
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16625
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).