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验证数据展示

  • WB analysis using 80545-1-RR

    WB analysis using 80545-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 80545-1-RR

    IF Staining of MCF-7 using 80545-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IP experiment of HUVEC using 80545-1-RR

    IP experiment of HUVEC using 80545-1-RR

    IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug.

  • Affinity and Kinetic Characterization of 80545-1-RR

    Affinity and Kinetic Characterization of 80545-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

Occludin Recombinant antibody

Occludin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 80545-1-RR

产品说明书

CloneNo. 8B2

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Pig

应用

WB, IF/ICC, IP, ELISA

别名

OCLN, 8B2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HaCaT cells, pig colon tissue, mouse colon tissue, HUVEC cells, U2OS cells
Positive IP detected inHUVEC cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80545-1-RR targets Occludin in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, Mouse, Pig
免疫原 Occludin fusion protein Ag26173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 occludin
别名 OCLN, 8B2
计算分子量 522 aa, 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC029886
基因名称 Occludin
Gene ID (NCBI) 4950
RRIDAB_2918901
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16625
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

实验方案

Product Specific Protocols
WB protocol for Occludin antibody 80545-1-RRDownload protocol
IF protocol for Occludin antibody 80545-1-RRDownload protocol
IP protocol for Occludin antibody 80545-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols