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验证数据展示

  • ChIP experiment of K-562 using 82712-3-RR

    ChIP experiment of K-562 using 82712-3-RR

    Chromatin was prepared from K-562 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of YY1 Antibody (82712-3-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR

  • WB analysis using 82712-3-RR

    WB analysis using 82712-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82712-3-RR (YY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82712-3-RR

    IF Staining of HepG2 using 82712-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 82712-3-RR

    IF Staining of HepG2 using 82712-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 82712-3-RR

    Affinity and Kinetic Characterization of 82712-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82712-3-RR against Human YY1 were performed. The affinity constant is 0.835 nM.

YY1 Recombinant antibody

YY1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ChIP, ELISA
Cat No. 82712-3-RR

产品说明书

CloneNo. 1B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ChIP, ELISA

别名

1B5, DELTA, Delta transcription factor, INO 80S, INO80S

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHepG2 cells
Positive ChIP detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Chromatin immunoprecipitation (ChIP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82712-3-RR targets YY1 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ChIP, ELISA Application Description
经测试反应性 human, mouse
免疫原 YY1 fusion protein Ag17792 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YY1 transcription factor
别名 1B5, DELTA, Delta transcription factor, INO 80S, INO80S
计算分子量 414 aa, 45 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC037308
基因名称 YY1
Gene ID (NCBI) 7528
RRIDAB_3086514
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25490
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

实验方案

Product Specific Protocols
WB protocol for YY1 antibody 82712-3-RRDownload protocol
IF protocol for YY1 antibody 82712-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols