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YY1 Recombinant antibody

YY1 Recombinant Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B5

Cat No : 82712-3-RR

Print datasheet

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

验证数据展示

  • WB analysis using 82712-3-RR

    WB analysis using 82712-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82712-3-RR (YY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82712-3-RR

    IF Staining of HepG2 using 82712-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82712-3-RR targets YY1 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen YY1 fusion protein Ag17792 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name YY1 transcription factor
Synonyms DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor
Calculated Molecular Weight 414 aa, 45 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

实验方案

Product Specific Protocols
WB protocol for YY1 antibody 82712-3-RRDownload protocol
IF protocol for YY1 antibody 82712-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols