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Albumin Recombinant antibody

Albumin Recombinant Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4K1

Cat No : 82783-6-RR

Print datasheet

Synonyms

ALB, albumin, DKFZp779N1935, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341

验证数据展示

  • WB analysis using 82783-6-RR

    WB analysis using 82783-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82783-6-RR (Albumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse liver using 82783-6-RR

    IF Staining of mouse liver using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 82783-6-RR

    IF Staining of mouse liver using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma, HepG2 cells
Positive IF detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82783-6-RR targets Albumin in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Albumin fusion protein Ag9569 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name albumin
Synonyms ALB, albumin, DKFZp779N1935, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341
Calculated Molecular Weight 609 aa, 69 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC034023
Gene Symbol ALB
Gene ID (NCBI) 213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

实验方案

Product Specific Protocols
WB protocol for Albumin antibody 82783-6-RRDownload protocol
IF protocol for Albumin antibody 82783-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols