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验证数据展示

  • FC experiment of A431 using CL488-66690

    FC experiment of A431 using CL488-66690

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone:1C1G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of A431 using CL488-66690

    IF Staining of A431 using CL488-66690

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 FKBP8 antibody (CL488-66690, Clone: 1C1G12 ) at dilution of 1:200, CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FKBP8 Monoclonal antibody

FKBP8 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66690

产品说明书

CloneNo. 1C1G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

FK506 binding protein 8, FK506-binding protein 8, FKBP 38, FKBP 8, FKBP38

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 66690-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66690 targets FKBP8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1649

Product name: Recombinant human FKBP8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-355 aa of BC009966

Sequence: MGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKGQVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYGPQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLAANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FK506 binding protein 8, 38kDa
别名 FK506 binding protein 8, FK506-binding protein 8, FKBP 38, FKBP 8, FKBP38
计算分子量 45 kDa
观测分子量 53 kDa
GenBank蛋白编号BC009966
基因名称 FKBP8
Gene ID (NCBI) 23770
RRIDAB_3084266
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14318
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FKBP8 antibody CL488-66690Download protocol
IF protocol for CL Plus 488 FKBP8 antibody CL488-66690Download protocol
Standard Protocols
Click here to view our Standard Protocols
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