验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue, rat brain tissue |
| Positive FC (Intra) detected in | U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-26975 targets NeuN in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag25689 Product name: Recombinant human NeuN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of NM_001082575 Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hexaribonucleotide binding protein 3 |
| 别名 | Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen |
| 观测分子量 | 46-52 kDa |
| GenBank蛋白编号 | NM_001082575 |
| 基因名称 | NeuN |
| Gene ID (NCBI) | 146713 |
| RRID | AB_2919878 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A6NFN3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Function
NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).
Tissue specificity
NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).
Involvement in disease
· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).
Isoforms
There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).
Post-translational modifications
Currently not known.
Cellular localization
NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 NeuN antibody CL594-26975 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
