验证数据展示
经过测试的应用
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-65141 targets CD4 in IF/ICC, FC applications and shows reactivity with Mouse samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 经测试反应性 | Mouse |
| 免疫原 | BALB/c mouse thymocytes 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD4 antigen |
| 别名 | Cd4, CD4 antigen, L3T4, Ly 4 |
| GenBank蛋白编号 | BC039137 |
| 基因名称 | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_2883707 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 CD4 antibody CL647-65141 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |