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Recombinant Human ACE protein (rFc Tag)

ED50

/

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

1636

Accession

P12821-1

Cat No : Eg2298

Print datasheet

Synonyms

ACE,ACE1,Angiotensin converting enzyme,Angiotensin

验证数据展示

  • Purity of Recombinant Human ACE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ACE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human ACE protein Leu30-Arg1256 (Accession# P12821-1) with a rabbit IgG Fc tag at the C-terminus.
Predicted Molecular Mass 167.2 kDa
SDS-PAGE 140-220 kDa, reducing (R) condition
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

References:

1.Bernstein,K.E.et al.(2012) Pharmacol.Rev.65,1-46.
2.Gribouval,O.et al.(2011) Hum.Mut.33,316-326.
3.Danilov,S.M.et al.(2010) J.Proteome Res.9,5782-5793.
4.Danilov,S.M.et al.(2018) Mol.Genet.Metab.123,501-510.
5.Kozuch,A.J.et al.(2023) Biomedicines.11,953.