Recombinant Human ACE protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
纯度 | >90 %, SDS-PAGE |
内毒素 | <0.1 EU/μg protein, LAL method |
生物活性 | Not tested |
来源 | HEK293-derived Human ACE protein Leu30-Arg1256 (Accession# P12821-1) with a rabbit IgG Fc tag at the C-terminus. |
基因ID | 1636 |
蛋白编号 | P12821-1 |
预测分子量 | 170.4 kDa |
SDS-PAGE | 140-200 kDa, reducing (R) conditions |
组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).
参考文献:
1.Bernstein,K.E.et al.(2012) Pharmacol.Rev.65,1-46. 2.Gribouval,O.et al.(2011) Hum.Mut.33,316-326. 3.Danilov,S.M.et al.(2010) J.Proteome Res.9,5782-5793. 4.Danilov,S.M.et al.(2018) Mol.Genet.Metab.123,501-510. 5.Kozuch,A.J.et al.(2023) Biomedicines.11,953.