6*his,His tag Polyclonal antibody

6*his,His tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10001-0-AP

Print datasheet

Synonyms

6 his, 6*his,His tag, his tag



经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

产品信息

10001-0-AP targets 6*his,His tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, ELISA
Cited Applications IF, IP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, silkworm
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name 6*his,His tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.

发表文章

SpeciesApplicationTitle
WB

Cell Death Dis

TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability.

Authors - Sha Zhou
WB

Cell Death Dis

KAT2A-mediated AR translocation into nucleus promotes abiraterone-resistance in castration-resistant prostate cancer.

Authors - Dingheng Lu
WB

Protein J

Crystal structure of Human ASB9-2 and substrate-recognition of CKB.

Authors - Fei Xiangwei X
WB

J Vet Diagn Invest

Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein.

Authors - Miaomiao Yan

Arch Insect Biochem Physiol

The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori.

Authors - Yafei Ma
silkwormWB

Arch Insect Biochem Physiol

The acetylation modification regulates the stability of Bm30K-15 protein and its mechanism in silkworm, Bombyx mori.

Authors - Jiao Lv