6*his,His tag Polyclonal antibody

6*his,His tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


recombinant protein and More (2)


WB, ELISA and More (2)



Cat No : 10001-0-AP

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6 his, 6*his,His tag, his tag


Positive WB detected inRecombinant protein


Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery


10001-0-AP targets 6*his,His tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, ELISA
Cited Applications IF, IP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, silkworm
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name 6*his,His tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.



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