6*his,His tag Polyclonal antibody
6*his,His tag Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (2)
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
产品信息
10001-0-AP targets 6*his,His tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, ELISA |
| Cited Applications | IF, IP, WB |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, silkworm |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein |
| Full Name | 6*his,His tag |
| Calculated molecular weight | 1 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability. | ||
Cell Death Dis KAT2A-mediated AR translocation into nucleus promotes abiraterone-resistance in castration-resistant prostate cancer. | ||
J Vet Diagn Invest Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein. | ||
Arch Insect Biochem Physiol The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori. | ||
Arch Insect Biochem Physiol The acetylation modification regulates the stability of Bm30K-15 protein and its mechanism in silkworm, Bombyx mori. |