验证数据展示
经过测试的应用
| Positive WB detected in | recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10001-0-AP targets 6*His, His-tag in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 6*His, His Tag |
| 别名 | his tag, his, 6*his,His tag, 6*His, 6 his |
| 计算分子量 | 1 kDa |
| 基因名称 | His Tag |
| Gene ID (NCBI) | 5 |
| RRID | AB_11232228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for 6*His, His-tag antibody 10001-0-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. | ||
Cell Death Differ SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress | ||
Nat Commun Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery. | ||
Nat Commun Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome. |