验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14266-1-AP targets ADRA2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ADRA2A fusion protein Ag5562 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adrenergic, alpha-2A-, receptor |
| 别名 | Alpha 2A adrenoreceptor, Alpha 2A adrenoceptor, Alpha 2A adrenergic receptor, ADRAR, ADRA2R |
| 计算分子量 | 49 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC050414 |
| 基因名称 | ADRA2A |
| Gene ID (NCBI) | 150 |
| RRID | AB_2636822 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08913 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADRA2A (Adrenoceptor Alpha-2) belongs to the adrenoceptor alpha-2 family of GPCRs coupled to the inhibitory G-alpha subunit (Gi) that suppresses adenylyl cyclase activity, thereby suppressing the cyclization of AMP to cAMP (PMID: 38132444). ADRA2A inhibits the flow of sympathetic nerves in the central nervous system and plays a major role in sedation, anxiolysis, and antinociception (PMID: 33915198).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADRA2A antibody 14266-1-AP | Download protocol |
| IHC protocol for ADRA2A antibody 14266-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Dexmedetomidine facilitates autophagic flux to promote liver regeneration by suppressing GSK3β activity in mouse partial hepatectomy | ||
Int J Mol Sci Chronic Administrations of Guanfacine on Mesocortical Catecholaminergic and Thalamocortical Glutamatergic Transmissions. | ||
Am J Cancer Res SNHG15, a p53-regulated lncRNA, suppresses cisplatin-induced apoptosis and ROS accumulation through the miR-335-3p/ZNF32 axis. | ||
Front Immunol β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis. | ||
Exp Neurol Two-hour acute restraint stress facilitates escape behavior and learning outcomes through the activation of the Cdk5/GR P S211 pathway in male mice. |