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AGR2 Monoclonal antibody, PBS Only

AGR2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A8A8

Cat no : 66768-1-PBS

Print datasheet

Synonyms

GOB 4, AGR 2, AG-2, AG2, AG 2

验证数据展示

  • WB analysis of pig stomach using 66768-1-Ig (same clone as 66768-1-PBS)

    WB analysis of pig stomach using 66768-1-Ig (same clone as 66768-1-PBS)

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGR2 antibody included
  • IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGR2 antibody included
  • IF Staining of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    IF Staining of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • FC experiment of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    FC experiment of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human AGR2 (66768-1-Ig, Clone:1A8A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AGR2 Ready-To-Use IHC Kit. AGR2 primary antibody included.

产品信息

66768-1-PBS targets AGR2 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen AGR2 fusion protein Ag2919 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name anterior gradient homolog 2 (Xenopus laevis)
Synonyms GOB 4, AGR 2, AG-2, AG2, AG 2
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC015503
Gene Symbol AGR2
Gene ID (NCBI) 10551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95994
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.