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AGR2 Monoclonal antibody, PBS Only

AGR2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1A8A8

Cat No : 66768-1-PBS

Print datasheet

Synonyms

AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2

验证数据展示

  • WB analysis of pig stomach using 66768-1-Ig (same clone as 66768-1-PBS)

    WB analysis of pig stomach using 66768-1-Ig (same clone as 66768-1-PBS)

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGR2 antibody included
  • IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGR2 antibody included
  • FC experiment of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    FC experiment of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human AGR2 (66768-1-Ig, Clone:1A8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    IF Staining of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AGR2 Ready-To-Use IHC Kit. AGR2 primary antibody included.

产品信息

66768-1-PBS targets AGR2 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, Pig
Immunogen AGR2 fusion protein Ag2919 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name anterior gradient homolog 2 (Xenopus laevis)
Synonyms AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC015503
Gene Symbol AGR2
Gene ID (NCBI) 10551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95994
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.