验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells |
Positive IF/ICC detected in | HepG2 cells, A431 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67909-1-Ig targets AlaRS in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | AlaRS fusion protein Ag11151 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | alanyl-tRNA synthetase |
Synonyms | AARS, AARS1, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS |
Calculated Molecular Weight | 968 aa, 107 kDa |
Observed Molecular Weight | 107 kDa |
GenBank Accession Number | BC011451 |
Gene Symbol | AlaRS |
Gene ID (NCBI) | 16 |
RRID | AB_2918664 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P49588 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).
实验方案
Product Specific Protocols | |
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WB protocol for AlaRS antibody 67909-1-Ig | Download protocol |
IF protocol for AlaRS antibody 67909-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |