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AlaRS Monoclonal antibody

AlaRS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C8H3

Cat no : 67909-1-Ig

Print datasheet

Synonyms

AARS, AARS1, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS



经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IF/ICC detected inHepG2 cells, A431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67909-1-Ig targets AlaRS in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen AlaRS fusion protein Ag11151 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alanyl-tRNA synthetase
Synonyms AARS, AARS1, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight107 kDa
GenBank Accession NumberBC011451
Gene Symbol AlaRS
Gene ID (NCBI) 16
RRIDAB_2918664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

实验方案

Product Specific Protocols
WB protocol for AlaRS antibody 67909-1-IgDownload protocol
IF protocol for AlaRS antibody 67909-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols