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  • Featured Product
  • KD/KO Validated

AlaRS Monoclonal antibody, PBS Only

AlaRS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8H3

Cat no : 67909-1-PBS

Print datasheet

Synonyms

AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

验证数据展示

  • WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

    WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67909-1-Ig (same clone as 67909-1-PBS)

    WB analysis of HeLa using 67909-1-Ig (same clone as 67909-1-PBS)

    WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67909-1-Ig (same clone as 67909-1-PBS)

    IF Staining of HepG2 using 67909-1-Ig (same clone as 67909-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

产品信息

67909-1-PBS targets AlaRS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen AlaRS fusion protein Ag11151 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alanyl-tRNA synthetase
Synonyms AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight107 kDa
GenBank Accession NumberBC011451
Gene Symbol AlaRS
Gene ID (NCBI) 16
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49588
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).