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验证数据展示

  • WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

    WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67909-1-Ig (same clone as 67909-1-PBS)

    WB analysis of HeLa using 67909-1-Ig (same clone as 67909-1-PBS)

    WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67909-1-Ig (same clone as 67909-1-PBS)

    IF Staining of HepG2 using 67909-1-Ig (same clone as 67909-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

AlaRS Monoclonal antibody, PBS Only

AlaRS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67909-1-PBS

产品说明书

CloneNo. 1C8H3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, Indirect ELISA

别名

AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67909-1-PBS targets AlaRS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 AlaRS fusion protein Ag11151 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alanyl-tRNA synthetase
别名 AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS
计算分子量 968 aa, 107 kDa
观测分子量107 kDa
GenBank蛋白编号BC011451
基因名称 AlaRS
Gene ID (NCBI) 16
RRIDAB_2918664
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49588
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).