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验证数据展示

  • WB analysis using 67078-1-Ig

    WB analysis using 67078-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 67078-1-Ig

    WB analysis of human skeletal muscle using 67078-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67078-1-Ig (Alpha sarcoglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 67078-1-Ig

    IHC staining of mouse skeletal muscle using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SGCA antibody included
  • IHC staining of mouse skeletal muscle using 67078-1-Ig

    IHC staining of mouse skeletal muscle using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SGCA antibody included
  • IF Staining of mouse heart using 67078-1-Ig

    IF Staining of mouse heart using 67078-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse heart using 67078-1-Ig

    IF Staining of mouse heart using 67078-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Alpha Sarcoglycan antibody (67078-1-Ig, Clone: 2B10H7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse skeletal muscle using 67078-1-Ig

    IHC staining of mouse skeletal muscle using 67078-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67078-1-Ig (Alpha Sarcoglycan antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SGCA antibody included

Alpha Sarcoglycan Monoclonal antibody

Alpha Sarcoglycan Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67078-1-Ig

产品说明书

CloneNo. 2B10H7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

SGCA, SCARMD1, LGMD2D, Alpha-SG, Alpha-sarcoglycan

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human heart tissue, pig heart tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human liver tissue, mouse heart tissue, mouse tongue tissue, rat heart tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue, mouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy SGCA Ready-To-Use IHC Kit. SGCA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67078-1-Ig targets Alpha Sarcoglycan in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag28800

Product name: Recombinant human SGCA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-289 aa of BC025702

Sequence: QTTLHPLVGRVFVHTLDHETFLSLPEHVAVPPAVHITYHAHLQGHPDLPRWLRYTQRSPHHPGFLYGSATPEDRGLQVIEVTAYNRDSFDTTRQRLVLEIGDPEGPLLPYQAEFLVRSHDAEEVLPSTPASRFLSALGGLWEPGELQLLNVTSALDRGGRVPLPIEGRKEGVYIKVGSASPFSTCLKMVASPDSHARCAQGQPPLLSCYDTLAPHFRVDWCNVTLVDKSVPEPADEVPTPGDGILEHDPFFCPPTEAPDRDFLVD

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
别名 SGCA, SCARMD1, LGMD2D, Alpha-SG, Alpha-sarcoglycan
计算分子量 387 aa, 43 kDa
观测分子量 50 kDa
GenBank蛋白编号BC025702
基因名称 alpha Sarcoglycan
Gene ID (NCBI) 6442
RRIDAB_2882386
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.

实验方案

Product Specific Protocols
WB protocol for Alpha Sarcoglycan antibody 67078-1-IgDownload protocol
IHC protocol for Alpha Sarcoglycan antibody 67078-1-IgDownload protocol
IF protocol for Alpha Sarcoglycan antibody 67078-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols