验证数据展示
经过测试的应用
| Positive IHC detected in | human pancreas tissue, mouse pancreas tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22305-1-AP targets Amylin in IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, rat, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | islet amyloid polypeptide |
| 别名 | Amylin, DAP, Diabetes associated peptide, IAP, IAPP, islet amyloid polypeptide |
| 计算分子量 | 10 kDa |
| GenBank蛋白编号 | NM_000415 |
| 基因名称 | Amylin |
| Gene ID (NCBI) | 3375 |
| RRID | AB_2879067 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P10997 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IAPP, also known as Amylin, is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring a tumor of the pancreas that is derived from beta cells and secretes INS. IAPP is co-secreted with INS by pancreatic β-cells and largely contributes to glycemic control. Studies suggest that this protein, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Amylin antibody 22305-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
