APP Polyclonal antibody

APP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25524-1-AP

Print datasheet

Synonyms

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), amyloid beta 1-42, Amyloid beta A4 protein, amyloid P, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, Aβ1-42, beta Amyloid, C31, C80, C83, C99, CTFgamma, CVAP, Gamma CTF(50) C31, Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59,, P3(40), PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta



经过测试的应用

Positive WB detected inSH-SY5Y cells, 3T3-L1 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 25524-1-AP is APP Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APP fusion protein Ag22408
Full Name amyloid beta (A4) precursor protein
Synonyms A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), amyloid beta 1-42, Amyloid beta A4 protein, amyloid P, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, Aβ1-42, beta Amyloid, C31, C80, C83, C99, CTFgamma, CVAP, Gamma CTF(50) C31, Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59,, P3(40), PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta
Observed molecular weight 100 kDa
GenBank accession numberBC065529
Gene symbol APP
UniprotP05067
Gene ID (NCBI) 351
RRIDAB_2880118
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.

实验方案

Product Specific Protocols
WB protocol for APP antibody 25524-1-APDownload protocol
IHC protocol for APP antibody 25524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Neurosci Bull

PMID: 30632006

Authors - Yao Zhang
human,ratWB

Neurotherapeutics

Direct Activation of Protein Phosphatase 2A (PP2A) by Tricyclic Sulfonamides Ameliorates Alzheimer's Disease Pathogenesis in Cell and Animal Models.

Authors - Hui Wei
mouseIF

Aging (Albany NY)

Functional genomics study of protein inhibitor of activated STAT1 in mouse hippocampal neuronal cells revealed by RNA sequencing.

Authors - Kan He
mouseif

Acta Pharmacol Sin

Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function.

Authors - Xue Wang
ratWB

Evid Based Complement Alternat Med

Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.

Authors - Rong Fan
mouseWB

Int J Neurosci

Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice.

Authors - Yayun Du