BCS1L Polyclonal antibody
BCS1L Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, human brain tissue, mouse colon tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, mouse small intestine tissue, rat liver tissue |
Positive IHC detected in | human gliomas tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 7 publications below |
产品信息
10175-2-AP targets BCS1L in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | BCS1L fusion protein Ag0231 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | BCS1-like (yeast) |
Synonyms | BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC007500 |
Gene Symbol | BCS1L |
Gene ID (NCBI) | 617 |
RRID | AB_2259019 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y276 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BCS1L antibody 10175-2-AP | Download protocol |
IHC protocol for BCS1L antibody 10175-2-AP | Download protocol |
IF protocol for BCS1L antibody 10175-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Transl Med Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing. | ||
Hum Mol Genet Impaired complex III assembly associated with BCS1L gene mutations in isolated mitochondrial encephalopathy. | ||
Hum Mutat Cellular pathophysiological consequences of BCS1L mutations in mitochondrial complex III enzyme deficiency. | ||
Cell Rep Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. | ||
Biochim Biophys Acta LYRM7/MZM1L is a UQCRFS1 chaperone involved in the last steps of mitochondrial Complex III assembly in human cells. |