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  • Featured Product
  • KD/KO Validated

CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 15112-1-AP

Print datasheet

Synonyms

GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1

验证数据展示

  • IF Staining of HepG2 using 15112-1-AP

    IF Staining of HepG2 using 15112-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 15112-1-AP

    IP experiment of HeLa using 15112-1-AP

    IP result of anti-CAPRIN1 (IP:15112-1-AP, 4ug; Detection:15112-1-AP 1:5000) with HeLa cells lysate 1600 ug.

  • IF Staining of OC2 cells using 15112-1-AP

    IF Staining of OC2 cells using 15112-1-AP

    IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with OC2 cells by Dr. Emily Towers.

  • IF Staining of HeLa using 15112-1-AP

    IF Staining of HeLa using 15112-1-AP

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human breast cancer using 15112-1-AP

    IHC staining of human breast cancer using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPRIN1 antibody included
  • IHC staining of human breast cancer using 15112-1-AP

    IHC staining of human breast cancer using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPRIN1 antibody included
  • WB analysis using 15112-1-AP

    WB analysis using 15112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15112-1-AP

    WB analysis using 15112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15112-1-AP

    IHC staining of mouse testis using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPRIN1 antibody included
  • IHC staining of mouse testis using 15112-1-AP

    IHC staining of mouse testis using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPRIN1 antibody included
  • IF Staining of HeLa using 15112-1-AP

    IF Staining of HeLa using 15112-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of NIH/3T3 using 15112-1-AP

    FC experiment of NIH/3T3 using 15112-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (15112-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CAPRIN1 Ready-To-Use IHC Kit. CAPRIN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15112-1-AP targets CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, zebrafish
Immunogen CAPRIN1 fusion protein Ag7099 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell cycle associated protein 1
Synonyms GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC001731
Gene Symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2070016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

实验方案

Product Specific Protocols
WB protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IHC protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IF protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IP protocol for CAPRIN1 antibody 15112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
View Article
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
  • KD Validated
View Article
humanWB,IF

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
View Article
humanWB,IF

Nat Immunol

Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA.

Authors - Henan Xu
View Article
mouseWB,RIP

Nat Commun

Fear extinction is regulated by the activity of long noncoding RNAs at the synapse

Authors - Wei-Siang Liau
View Article
WB, IP

Neuron

CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.

Authors - Silvia De Rubeis
View Article