验证数据展示

IF Staining of HepG2 using 15112-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP experiment of HeLa using 15112-1-AP
IP result of anti-CAPRIN1 (IP:15112-1-AP, 4ug; Detection:15112-1-AP 1:5000) with HeLa cells lysate 1600 ug.
IF Staining of OC2 cells using 15112-1-AP
IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with OC2 cells by Dr. Emily Towers.
IF Staining of HeLa using 15112-1-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IHC staining of human breast cancer using 15112-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CAPRIN1 antibody included
IHC staining of human breast cancer using 15112-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CAPRIN1 antibody included
WB analysis using 15112-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 15112-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 15112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CAPRIN1 antibody included
IHC staining of mouse testis using 15112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CAPRIN1 antibody included
IF Staining of HeLa using 15112-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of NIH/3T3 using 15112-1-AP
1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (15112-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO验证

CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15112-1-AP

产品说明书

引用文献(80)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (80)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections
Positive FC (Intra) detected in	NIH/3T3 cells

Planning an IHC experiment? We recommend our IHCeasy CAPRIN1 Ready-To-Use IHC Kit. CAPRIN1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 8 publications below
WB	See 57 publications below
IHC	See 3 publications below
IF	See 30 publications below
IP	See 6 publications below
CoIP	See 1 publications below
RIP	See 2 publications below

产品信息

15112-1-AP targets CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, RIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, rabbit, canine, zebrafish
免疫原	CAPRIN1 fusion protein Ag7099 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cell cycle associated protein 1
别名	GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1
计算分子量	78 kDa
观测分子量	116 kDa
GenBank蛋白编号	BC001731
基因名称	CAPRIN1
Gene ID (NCBI)	4076
RRID	AB_2070016
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q14444
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

实验方案

Product Specific Protocols
WB protocol for CAPRIN1 antibody 15112-1-AP	Download protocol
IHC protocol for CAPRIN1 antibody 15112-1-AP	Download protocol
IF protocol for CAPRIN1 antibody 15112-1-AP	Download protocol
IP protocol for CAPRIN1 antibody 15112-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Authors - Lei Sun View Article
human	WB	Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Authors - Peiguo Yang KD Validated View Article
human	WB,IF	Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. Authors - David W Sanders View Article
human	WB,IF	Nat Immunol Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA. Authors - Henan Xu View Article
mouse	WB,RIP	Nat Commun Fear extinction is regulated by the activity of long noncoding RNAs at the synapse Authors - Wei-Siang Liau View Article
	WB, IP	Neuron CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation. Authors - Silvia De Rubeis View Article

验证数据展示

IF Staining of HepG2 using 15112-1-AP

IP experiment of HeLa using 15112-1-AP

IF Staining of OC2 cells using 15112-1-AP

IF Staining of HeLa using 15112-1-AP

IHC staining of human breast cancer using 15112-1-AP

IHC staining of human breast cancer using 15112-1-AP

WB analysis using 15112-1-AP

WB analysis using 15112-1-AP

IHC staining of mouse testis using 15112-1-AP

IHC staining of mouse testis using 15112-1-AP

IF Staining of HeLa using 15112-1-AP

FC experiment of NIH/3T3 using 15112-1-AP

CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA.

Fear extinction is regulated by the activity of long noncoding RNAs at the synapse

CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.