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验证数据展示

  • IF Staining of HeLa using CL488-15112

    IF Staining of HeLa using CL488-15112

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CAPRIN1 antibody (CL488-15112) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-15112

    FC experiment of HeLa using CL488-15112

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CAPRIN1 (CL488-15112) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15112

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CAPRIN1 Ready-To-Use IHC Kit. CAPRIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15112-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15112 targets CAPRIN1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CAPRIN1 fusion protein Ag7099 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell cycle associated protein 1
别名 GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1
计算分子量 78 kDa
观测分子量 116 kDa
GenBank蛋白编号BC001731
基因名称 CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2919110
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14444
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CAPRIN1 antibody CL488-15112Download protocol
FC protocol for CL Plus 488 CAPRIN1 antibody CL488-15112Download protocol
Standard Protocols
Click here to view our Standard Protocols