Caspase 2/p32/p18 Polyclonal antibody

Caspase 2/p32/p18 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10436-1-AP

Print datasheet

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1



经过测试的应用

Positive WB detected inStaurosporine treated Jurkat cells, HEK-293 cells, HeLa cells, HL-60 cells, Jurkat cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10436-1-AP targets Caspase 2/p32/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsELISA, IF, RIP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rabbit
Immunogen Caspase 2/p32/p18 fusion protein Ag0694 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 2, apoptosis-related cysteine peptidase
Synonyms CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1
Calculated Molecular Weight 452 aa, 51 kDa
Observed Molecular Weight48-51 kDa, 32 kDa, 18kDa
GenBank Accession NumberBC002427
Gene Symbol CASP2
Gene ID (NCBI) 835
RRIDAB_2228450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42575
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

实验方案

Product Specific Protocols
WB protocol for Caspase 2/p32/p18 antibody 10436-1-APDownload protocol
IHC protocol for Caspase 2/p32/p18 antibody 10436-1-APDownload protocol
IP protocol for Caspase 2/p32/p18 antibody 10436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Front Microbiol

Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.

Authors - Bei Wang

Front Pharmacol

PMID: 32210806

Authors - Wen Su
rabbitRIP

Life Sci

Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro.

Authors - Guo-Jian Jiang
humanELISA

Toxicol In Vitro

PMID: 32320760

Authors - Ting Yang
mouseWB

Toxicon

Involvement of caspase in patulin-induced hepatotoxicity in vitro and in vivo.

Authors - Baigang Zhang
mouseWB

Brain Res

miR-1247-3p mediates apoptosis of cerebral neurons by targeting caspase-2 in stroke.

Authors - Rong Zhang