CoraLite® Plus 488 Anti-Human CD107a / LAMP1 (H4A3)
CD107a / LAMP1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
H4A3
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65051-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65051 targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | Human |
| Immunogen | Human adherent peripheral blood cells 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lysosomal-associated membrane protein 1 |
| Synonyms | CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker |
| GenBank Accession Number | BC006345 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_2883149 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P11279 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-65051 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
