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CoraLite® Plus 647 Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG1 Recombinant Antibody

CD107a / LAMP1 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4A3

Cat no : CL647-65519

Print datasheet

Synonyms

CD107a, LAMP 1, LAMP1, LAMPA, LGP120

验证数据展示

  • FC experiment of HeLa using CL647-65519

    FC experiment of HeLa using CL647-65519

    1x10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107a Mouse Recombinant Antibody (CL647-65519, Clone:H4A3) or Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using CL647-65519

    FC experiment of HeLa using CL647-65519

    1x10^6 HeLa cells were intracellularly stained with 5 ul Anti-Human CD107a Mouse Recombinant Antibody (CL647-65519, Clone:H4A3) or Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65519-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65519 targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Human adherent peripheral blood cells 种属同源性预测
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms CD107a, LAMP 1, LAMP1, LAMPA, LGP120
GenBank Accession NumberBC006345
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD107a / LAMP1 antibody CL647-65519Download protocol
Standard Protocols
Click here to view our Standard Protocols