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CoraLite® Plus 555 Anti-Human CD107a / LAMP1 (H4A3)

CD107a / LAMP1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

H4A3

Cat no : CL555-65051

Print datasheet

Synonyms

LAMP1, CD107a, H4A3, LAMP 1, CD107 antigen-like family member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1

验证数据展示

  • FC experiment of human peripheral blood platelets using CL555-65051

    FC experiment of human peripheral blood platelets using CL555-65051

    1X10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD107a / LAMP1 (CL555-65051, Clone:H4A3) (red) or Mouse IgG1 Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood platelets
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65051-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65051 targets CD107a / LAMP1 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human adherent peripheral blood cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms LAMP1, CD107a, H4A3, LAMP 1, CD107 antigen-like family member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1
GenBank Accession NumberBC006345
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11279
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD107a / LAMP1 antibody CL555-65051Download protocol
Standard Protocols
Click here to view our Standard Protocols