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CD11b Recombinant antibody, PBS Only

CD11b Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

ICRF44

Cat no : 65582-1-PBS

Print datasheet

Synonyms

ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha

验证数据展示

  • FC experiment of human PBMCs using 65582-1-MR (same clone as 65582-1-PBS)

    FC experiment of human PBMCs using 65582-1-MR (same clone as 65582-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody (65582-1-MR, Clone:ICRF44) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65582-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65582-1-MR (same clone as 65582-1-PBS)

    FC experiment of human PBMCs using 65582-1-MR (same clone as 65582-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody (65582-1-MR, Clone:ICRF44) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65582-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.

产品信息

65582-1-PBS targets CD11b in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Synonyms ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha
Calculated Molecular Weight 1152 aa, 127 kDa
GenBank Accession NumberBC096346
Gene Symbol CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).