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Anti-Mouse CD16 / CD32 (2.4G2)

CD16 / CD32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

2.4G2

Cat No : 65080-1-Ig

Print datasheet

Synonyms

AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

验证数据展示

  • FC experiment of mouse splenocytes using 65080-1-Ig

    FC experiment of mouse splenocytes using 65080-1-Ig

    1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16 / CD32 (65080-1-Ig, Clone: 2.4G2) and FITC Anti-Rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and FITC Anti-Rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

产品信息

65080-1-Ig targets CD16 / CD32 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Full Name Fc receptor, IgG, low affinity IIb
Synonyms AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1
GenBank accession numberBC038070
Gene symbol Fcgr2b
UniprotP08101
Gene ID (NCBI) 14130
RRIDAB_2918383
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C.

背景介绍

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

实验方案

Product Specific Protocols
FC protocol for CD16 / CD32 antibody 65080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols