Anti-Mouse CD16 / CD32 (2.4G2)
CD16 / CD32 Monoclonal Antibody for FC (Intra)
Host / Isotype
Rat / IgG2b, kappa
Reactivity
mouse
Applications
FC (Intra) and More (1)
Conjugate
Unconjugated
CloneNo.
2.4G2
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | mouse splenocytes |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 1 publications below |
产品信息
65080-1-Ig targets CD16 / CD32 in FC (Intra) applications and shows reactivity with mouse samples.
| Tested Applications | FC (Intra) Application Description |
| Cited Applications | FC |
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Fc receptor, IgG, low affinity IIb |
| Synonyms | CD32, 2.4G2, Fcr 2, FcgRII, Fcgr2b |
| GenBank Accession Number | BC038070 |
| Gene Symbol | CD16/32 |
| Gene ID (NCBI) | 14130 |
| RRID | AB_2918383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08101 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD16 / CD32 antibody 65080-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
