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验证数据展示

  • FC experiment of human PBMCs using CL594-65110

    FC experiment of human PBMCs using CL594-65110

    1X10^6 human PBMCs were surface stained with CL750 CD3 (OKT3) and 5 ul CoraLite® Plus 488 Anti-Human CD19 (CL488-65110, Clone:HIB19) or CoraLite® Plus 488 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CoraLite®594 Anti-Human CD19 (HIB19)

CD19 Monoclonal Antibody for FC
Cat No. CL594-65110

产品说明书

CloneNo. HIB19

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65110-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65110 targets CD19 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD19 molecule
别名 B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12
计算分子量 556 aa, 61 kDa
GenBank蛋白编号BC006338
基因名称 CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
RRIDAB_2919934
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Affinity purification
UNIPROT IDP15391
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

实验方案

Product Specific Protocols
FC protocol for CL594 CD19 antibody CL594-65110Download protocol
Standard Protocols
Click here to view our Standard Protocols