验证数据展示
经过测试的应用
| Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 3 publications below |
产品信息
APC-65137 targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples.
| Tested Applications | FC Application Description |
| Cited Applications | FC |
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Rat / IgG1, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 2 receptor, alpha chain |
| Synonyms | CD25, Il-2ra, Il2ra, IL-2R subunit alpha, Il2r |
| GenBank Accession Number | BC114437 |
| Gene Symbol | CD25 |
| Gene ID (NCBI) | 16184 |
| RRID | AB_2883018 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01590 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD25/IL-2RA antibody APC-65137 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma. | ||
Acta Biomater Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment | ||
J Neuroinflammation 27-hydroxycholesterol causes cognitive deficits by disturbing Th17/Treg balance and the related immune responses in mild cognitive impairment patients and C57BL/6J mice |