验证数据展示
经过测试的应用
Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 2 publications below |
产品信息
FITC-65137 targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples.
经测试应用 | FC Application Description |
文献引用应用 | FC |
经测试反应性 | mouse |
文献引用反应性 | mouse |
免疫原 | N/A 种属同源性预测 |
宿主/亚型 | Rat / IgG1, lambda |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | interleukin 2 receptor, alpha chain |
别名 | CD25, Il-2ra, Il2ra, IL-2R subunit alpha, Il2r |
GenBank蛋白编号 | BC114437 |
基因名称 | CD25 |
Gene ID (NCBI) | 16184 |
RRID | AB_2883797 |
偶联类型 | FITC Plus Fluorescent Dye |
最大激发/发射波长 | 495 nm / 524 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P01590 |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).
实验方案
Product Specific Protocols | |
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FC protocol for FITC Plus CD25/IL-2RA antibody FITC-65137 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Oncogene ZG16 enhances the maturation of dendritic cells via induction of CD40 and contributes to the antitumor immunity in pancreatic cancer | ||
Phytomedicine Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma. | ||
Cell Rep Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses |