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PE Anti-Mouse CD25 (PC61.5)

CD25 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, lambda

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

PC61.5

Cat no : PE-65137

Print datasheet

Synonyms

CD25, Il2r, Il2ra, Il-2ra, Ly 43

验证数据展示

  • FC experiment of mouse splenocytes using PE-65137

    FC experiment of mouse splenocytes using PE-65137

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Human CD4 and 0.5 ug PE Anti-Mouse CD25 (PE-65137, Clone:PC61.5) or 0.5 ug PE Rat IgG1 Isotype Control (HRPN) (PE-65212, Clone: HRPN). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG1, lambda
Class Monoclonal
Type Antibody
Full Name interleukin 2 receptor, alpha chain
Synonyms CD25, Il2r, Il2ra, Il-2ra, Ly 43
GenBank Accession NumberBC114437
Gene Symbol CD25
Gene ID (NCBI) 16184
RRIDAB_2883919
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01590
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for PE CD25 antibody PE-65137Download protocol
Standard Protocols
Click here to view our Standard Protocols