• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 647 Anti-Mouse CD25 (PC61.5)

CD25 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, lambda

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

PC61.5

Cat no : CL647-65137

Print datasheet

Synonyms

CD25, Il2r, Il2ra, Il-2ra, Ly 43

验证数据展示

  • FC experiment of mouse splenocytes using CL647-65137

    FC experiment of mouse splenocytes using CL647-65137

    1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD25 (CL647-65137, Clone: PC61.5) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65137-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG1, lambda
Class Monoclonal
Type Antibody
Full Name interleukin 2 receptor, alpha chain
Synonyms CD25, Il2r, Il2ra, Il-2ra, Ly 43
GenBank Accession NumberBC114437
Gene Symbol CD25
Gene ID (NCBI) 16184
RRIDAB_2883703
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01590
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD25 antibody CL647-65137Download protocol
Standard Protocols
Click here to view our Standard Protocols