CoraLite® Plus 750 Anti-Mouse CD25 (PC61.5)
CD25 Monoclonal Antibody for FC
Host / Isotype
Rat / IgG1, lambda
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
PC61.5
验证数据展示
经过测试的应用
| Positive FC detected in | plate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65137-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Mouse |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Rat / IgG1, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 2 receptor, alpha chain |
| Synonyms | CD25, Il2r, Il2ra, Ly 43 |
| GenBank Accession Number | BC114437 |
| Gene Symbol | CD25 |
| Gene ID (NCBI) | 16184 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01590 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD25 antibody CL750-65137 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
