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验证数据展示

  • WB analysis using 60181-1-Ig (same clone as 60181-1-PBS)

    WB analysis using 60181-1-Ig (same clone as 60181-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60181-1-Ig (CD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

    WB analysis of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

    WB result of CD3 antibody (60181-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells. This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD3 antibody included
  • IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

    IF Staining of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD3 antibody included
  • IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3), FOXP3 antibody (22228-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50222-1

    Cytometric bead array standard curve of MP50222-1

    Cytometric bead array standard curve of MP50222-1, CD3E Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60181-2-PBS. Detection antibody: 60181-1-PBS. Standard:Ag11797. Range: 0.098-50 ng/mL.

  • Featured Product
  • KD/KO验证

CD3 Monoclonal antibody, PBS Only (Detector)

CD3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 60181-1-PBS

产品说明书

CloneNo. 3F3A1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

产品信息

60181-1-PBS targets CD3 as part of a matched antibody pair:

MP50222-1: 60181-2-PBS capture and 60181-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD3 fusion protein Ag11797 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD3e molecule, epsilon (CD3-TCR complex)
别名 CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon
计算分子量 207 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC049847
基因名称 CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
RRIDAB_10859262
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07766
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.