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Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Antibody, PBS Only

CD73 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

AD2

Cat no : 65564-1-PBS

Print datasheet

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

验证数据展示

  • FC experiment of human PBMCs using 65564-1-PBS

    FC experiment of human PBMCs using 65564-1-PBS

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 Mouse Recombinant Antibody, PBS Only (65564-1-PBS, Clone:AD2) or 0.5 ug Mouse IgG2a Isotype Contro (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65564-1-PBS targets CD73 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Pre-B leukemia cell line 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name 5'-nucleotidase, ecto (CD73)
Synonyms 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE
Calculated Molecular Weight 29 kDa, 63 kDa
GenBank Accession NumberBC015940
Gene Symbol CD73
Gene ID (NCBI) 4907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21589
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
FC protocol for CD73 antibody 65564-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols