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- KD/KO Validated
CDR2L Polyclonal antibody
CDR2L Polyclonal Antibody for IF/ICC, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, IF, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CDR2L Ready-To-Use IHC Kit. CDR2L primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14563-1-AP targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, IF, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Immunogen | CDR2L fusion protein Ag6127 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cerebellar degeneration-related protein 2-like |
| Synonyms | CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC047534 |
| Gene Symbol | CDR2L |
| Gene ID (NCBI) | 30850 |
| RRID | AB_2076740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86X02 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm. The predicted MW of this protein is 53 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDR2L antibody 14563-1-AP | Download protocol |
| IHC protocol for CDR2L antibody 14563-1-AP | Download protocol |
| IF protocol for CDR2L antibody 14563-1-AP | Download protocol |
| IP protocol for CDR2L antibody 14563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis. | ||
Cancer Immunol Immunother Cerebellar degeneration-related proteins 2 and 2-like are present in ovarian cancer in patients with and without Yo antibodies. | ||
Ann Clin Transl Neurol Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration. | ||
J Neuroimmunol Expression of cerebellar degeneration-related proteins CDR2 and CDR2L in human and rat brain tissue. |
