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CDR2L Polyclonal antibody
CDR2L Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | human cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14563-1-AP targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat |
Immunogen | CDR2L fusion protein Ag6127 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cerebellar degeneration-related protein 2-like |
Synonyms | Paraneoplastic 62 kDa antigen, HUMPPA, Cerebellar degeneration-related protein 2-like |
Calculated Molecular Weight | 53 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC047534 |
Gene Symbol | CDR2L |
Gene ID (NCBI) | 30850 |
RRID | AB_2076740 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86X02 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CDR2L antibody 14563-1-AP | Download protocol |
IHC protocol for CDR2L antibody 14563-1-AP | Download protocol |
IF protocol for CDR2L antibody 14563-1-AP | Download protocol |
IP protocol for CDR2L antibody 14563-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Neuropathol Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis. | ||
Cancer Immunol Immunother Cerebellar degeneration-related proteins 2 and 2-like are present in ovarian cancer in patients with and without Yo antibodies. | ||
Ann Clin Transl Neurol Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration. | ||
J Neuroimmunol Expression of cerebellar degeneration-related proteins CDR2 and CDR2L in human and rat brain tissue. |