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CEBP Alpha/CEBPA Polyclonal antibody

CEBPA Polyclonal Antibody for WB, IP, ELISA
Cat No. 18311-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IP, ELISA and More (3)

C/EBP alpha, C/EBPα, CEBP, CEBPA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inL02 cells, human liver tissue
Positive IP detected inL02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18311-1-AP targets CEBPA in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF, chIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, hamster, sheep, goat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CCAAT/enhancer binding protein (C/EBP), alpha
别名 C/EBP alpha, C/EBPα, CEBP, CEBPA
计算分子量 38 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC160133
基因名称 CEBPA
Gene ID (NCBI) 1050
RRIDAB_2077892
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49715
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).

实验方案

Product Specific Protocols
WB protocol for CEBPA antibody 18311-1-APDownload protocol
IP protocol for CEBPA antibody 18311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
  • KD Validated
mouseWB

Exp Mol Med

Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury

Authors - Zhenjun Yu
ChIP

J Clin Invest

Dysregulation of PI3K and hippo signaling pathways synergistically induces chronic pancreatitis via Ctgf upregulation.

Authors - Takeshi Tamura
mouseChIP

Bone Res

mTORC1 induces plasma membrane depolarization and promotes preosteoblast senescence by regulating the sodium channel Scn1a.

Authors - Ajuan Chen
humanWB

Theranostics

PIWIL3/OIP5-AS1/miR-367-3p/CEBPA feedback loop regulates the biological behavior of glioma cells.

Authors - Xiaobai Liu
  • KD Validated
humanWB

Leukemia

Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells.

Authors - Ladan Kobari
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