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验证数据展示

  • WB analysis of HeLa using 66082-1-Ig (same clone as 66082-1-PBS)

    WB analysis of HeLa using 66082-1-Ig (same clone as 66082-1-PBS)

    WB result of CISD2 antibody (66082-1-Ig, 1:3000) with si-Control and si-CISD2 transfected HeLa cells. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

    IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • WB analysis of fetal human brain using 66082-1-Ig (same clone as 66082-1-PBS)

    WB analysis of fetal human brain using 66082-1-Ig (same clone as 66082-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

    IF Staining of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CISD2-Specific Monoclonal antibody, PBS Only

CISD2-Specific Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66082-1-PBS

产品说明书

CloneNo. 3D7A3

宿主/亚型

Mouse / IgG2b

种属反应性

human, zebrafish, rat, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66082-1-PBS targets CISD2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, zebrafish, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, zebrafish, rat, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CDGSH iron sulfur domain 2
别名 CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2
观测分子量 15 kDa
GenBank蛋白编号NM_001008388
基因名称 CISD2
Gene ID (NCBI) 493856
RRIDAB_11232425
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N5K1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.