• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CISD2-Specific Monoclonal antibody, PBS Only

CISD2-Specific Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, zebrafish, rat, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3D7A3

Cat No : 66082-1-PBS

Print datasheet

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

验证数据展示

  • WB analysis of HeLa using 66082-1-Ig (same clone as 66082-1-PBS)

    WB analysis of HeLa using 66082-1-Ig (same clone as 66082-1-PBS)

    WB result of CISD2 antibody (66082-1-Ig, 1:3000) with si-Control and si-CISD2 transfected HeLa cells. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

    IHC staining of human kidney using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • WB analysis of fetal human brain using 66082-1-Ig (same clone as 66082-1-PBS)

    WB analysis of fetal human brain using 66082-1-Ig (same clone as 66082-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

    IF Staining of MCF-7 using 66082-1-Ig (same clone as 66082-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66082-1-PBS in a different storage buffer formulation.

产品信息

66082-1-PBS targets CISD2-Specific in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, zebrafish, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, zebrafish, rat, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CDGSH iron sulfur domain 2
Synonyms CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2
Observed Molecular Weight 15 kDa
GenBank Accession NumberNM_001008388
Gene Symbol CISD2
Gene ID (NCBI) 493856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N5K1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.