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CYB5R3 Polyclonal antibody
CYB5R3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, MCF-7 cells, human lung tissue, human liver tissue, mouse liver tissue, human placenta tissue, HeLa cells, HepG2 cells, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10894-1-AP targets CYB5R3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig, hamster |
Immunogen | CYB5R3 fusion protein Ag1339 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cytochrome b5 reductase 3 |
Synonyms | NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1 |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 30-35 kDa |
GenBank Accession Number | BC004821 |
Gene Symbol | CYB5R3 |
Gene ID (NCBI) | 1727 |
RRID | AB_2292715 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P00387 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
IHC protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
IF protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
IP protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Transl Med Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes
| ||
J Cell Biol Dia1-dependent adhesions are required by epithelial tissues to initiate invasion. | ||
JCI Insight CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling | ||
Brain Struct Funct High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain. | ||
Blood Adv Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice. | ||
Free Radic Biol Med Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3 |