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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CYB5R3 Polyclonal antibody

CYB5R3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10894

Print datasheet

Synonyms

NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1

验证数据展示

  • IF Staining of HeLa using CL488-10894

    IF Staining of HeLa using CL488-10894

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CYB5R3 antibody (CL488-10894) at dilution of 1:200.

  • FC experiment of HeLa using CL488-10894

    FC experiment of HeLa using CL488-10894

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CYB5R3 (CL488-10894) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYB5R3 Ready-To-Use IHC Kit. CYB5R3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10894-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10894 targets CYB5R3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CYB5R3 fusion protein Ag1339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome b5 reductase 3
Synonyms NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
RRIDAB_2919008
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00387
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CYB5R3 antibody CL488-10894Download protocol
FC protocol for CL Plus 488 CYB5R3 antibody CL488-10894Download protocol
Standard Protocols
Click here to view our Standard Protocols